NGS SYK-6 RB-TnSeq library: Wx + 5 mM vanillate enrichment Replicate A - SRA

SRX18378034: NGS SYK-6 RB-TnSeq library: Wx + 5 mM vanillate enrichment Replicate A
1 ILLUMINA (Illumina HiSeq 3000) run: 10.3M spots, 1.5G bases, 571Mb downloads

Design: A 1 mL cell pellet was used to extract and purify gDNA. Specific primers amplified the transposon-associated barcode region with Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA). Samples were pooled and sent to Genewiz for sequencing.

Submitted by: National Renewable Energy Laboratory

Study: RB-TnSeq in Sphingobium sp. SYK-6

PRJNA905394 • SRP409783 • All experiments • All runs

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In this study, we generated a randomly barcoded transposon mutant library in Sphingobium sp. SYK-6 and applied it for characterization of lignin-related aromatic catabolism. TnSeq mapped unique barcoded transposon insertions to specific genomic loci, and then BarSeq enumerated the number of barcode insertions before and after various growth selection experiments.

Sample:

SAMN31871894 • SRS15864023 • All experiments • All runs

Organism: Sphingobium

Library:

Name: Wx_VA_cat_A

Instrument: Illumina HiSeq 3000

Strategy: Tn-Seq

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 10.3M spots, 1.5G bases, 571Mb

Run	# of Spots	# of Bases	Size	Published
SRR22408464	10,321,174	1.5G	571Mb	2023-01-31

ID:: 25397309

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